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KMID : 1094720070120040457
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Biotechnology and Bioprocess Engineering
2007 Volume.12 No. 4 p.457 ~ p.461
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Optimization of culture medium for rifamycin SV production by Amycolatopsis mediterranei MM2 using statistical designs
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Shin Hae-Joong
Kim Chang-Joon
Kim Sung-Bae
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Abstract
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In this paper, we conducted a statistical optimization of the medium components for the production of rifamycin SV byAmycolatopsis mediterranei MM2. In order to maximize the yield of rifamycin SV, a Plakett-Burman experimental design (PBD) was initially utilized in the screening of the medium components among 11 nutrients. Glycerol and yeast extract were determined to influence significantly the yield of rifamycin SV. Then, a central-composite experimental design (CCD) was utilized in order to optimize the concentrations of the screened components accqired using the PBD and to predict the mutual interactions occurring between the screened components. The predicted optimal glycerol and yeast extract concentrations were determined to be 43.8 and 9.5 g/L, respectively. At this optimum point, the predicted rifamycin SV yield was 490.5 mg/L, whereas the corresponding experimental yield was 480.3±43.8 mg/L.
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KEYWORD
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rifamycin SV, Amycolatopsis mediterranei, statistical optimization, experimental design
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